Trace elements essential for life are ones that generally occur in the body in micrograms per gram of tissue and are usually required by humans in milligrams per day amounts; these elements are copper, iron, manganese, and zinc. The evidence for essentiality for humans is substantial and not controversial for these elements. Specific biochemical functions have been found for each of these minerals. Because magnesium has many characteristics of a trace element, it also will be included in this group of elements. Another element that will be included is boron, because it is required to complete the life cycle of zebrafish and frogs, which suggests that it is essential for higher animals and humans.
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